Di-, tri- and tetramers of b-(1->3)-linked N-acetyllactosamine residues have been synthesized as their Me glycosides, to be used in isothermal titrn. calorimetry (ITC) binding studies to various galectins. The synthetic strategy involves two types of regioselective glycosylations: couplings of a galactosyl donor to 3,4-diol N-tetrachlorophthalimido glucose acceptors to give the lactosamine monomer building blocks, and subsequent formation of the oligomers through consecutive couplings of lactosamine donors to 2',3',4'-lactosamine acceptors, with high selectivity for the desired products. [on SciFinder (R)]